Authors: Rodrigo Covre Vieira1*, Jeniffer do Socorro Valente Monteiro2, Estéfane Primo Manso2, Maria Renata Mendonça dos Santos3, Mihoko Yamamoto Tsutsumi4, Edna Aoba Yassui Ishikawa1, Stephen Francis Ferrari5,Karla Valéria Batista Lima6 and Maísa Silva de Sousa1 Abstract Background Human papillomavirus (HPV) infection is associated with cervical cancer, the most frequent cancer in women from northern Brazil. Assessment […]
Global availability of data on HPV genotype distribution in cervical, vulvar and vaginal disease and genotype specific prevalence and incidence of HPV infection in females Authors: Monika Wagner1*, Liga Bennetts1, Harshila Patel1, Sharon Welner1, Silvia de Sanjose34 andThomas W Weiss2 Abstract (provisional) Background Country-level HPV genotyping data may be sought by decision-makers to gauge the genotype […]
By Bree Weaver MD, Marcia Shew MD, Brahim Qadadri BS, Wanzhu Tu PhD, Yang Tong PhD, Cheryl Denski BG, J. Dennis Fortenberry MD, Darron Brown MD Abstract Purpose The aim of the study was to better characterize the natural history of human papillomavirus (HPV) infections in female adolescents. Methods Female adolescents were enrolled in a […]
American Association for Cancer Research
Francesca M. Carozzi1, Maria L. Tornesello2, Elena Burroni1, Giovanna Loquercio2, Giuseppe Carillo3, Claudio Angeloni4, Aurora Scalisi5, Rosalba Macis6, Francesco Chini7, Franco M. Buonaguro2, Paolo Giorgi Rossi7 and for the HPV Prevalence Italian Working Group
Paolo Giorgi Rossi, Laziosanità, Via di Santa Costanza, 53, Rome 00198, Italy. E-mail: firstname.lastname@example.org
Background: The aim of this multicentric study was to identify human papillomavirus (HPV) type distribution in invasive cervical cancer and high-grade cervical intraepithelial neoplasia 2/3 (CIN2/3) in Italy.
Methods: Cases were sampled through the electronic databases at the pathology units of eight centers in six regions from central and southern Italy. HPV types were detected from paraffin-embedded tissue samples and cervical specimens through amplification of HPV DNA with GP5+/GP6+ primers, followed by genotyping with reverse line blot (RLB). Untyped HPV-positive samples were sequenced. HPV-negative samples underwent nested PCR, followed by either RLB or sequencing. Finally, the remaining HPV-negative samples were amplified with primers targeting the virus E6 to E7 regions.